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Antibodies certain to the blocking peptide no more bind to your epitope to the target protein. By comparing the staining in the blocked antibody vs . the antibody by itself, one can see which staining is particular.

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This guidebook outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best final results are...

This guideline outlines more doable optimization of cellular and immunoassay parameters to make certain the best possible benefits are...

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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...

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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.

Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic function. Cyclin here E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.

Overexpression of the gene has become observed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital job in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two added splice variants were being reported but detailed nucleotide sequence data just isn't but readily available.

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